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Literature summary for 3.2.1.151 extracted from
- A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme (1999), Glycobiology, 9, 93-100.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Aspergillus aculeatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.6 | - |
xyloglucan | pH 5, 37°C | Aspergillus aculeatus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23600 | - |
mass spectrometry | Aspergillus aculeatus |
| 34000 | - |
SDS-PAGE | Aspergillus aculeatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xyloglucan + H2O | Aspergillus aculeatus | - |
xyloglucan oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus aculeatus | O94218 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Aspergillus aculeatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 260 | - |
- |
Aspergillus aculeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xyloglucan + H2O | - |
Aspergillus aculeatus | xyloglucan oligosaccharides | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
optimally stable below | Aspergillus aculeatus |
| 50 | - |
80% loss of activity after 2h | Aspergillus aculeatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 113 | - |
xyloglucan | from tamarind, pH 5, 37°C | Aspergillus aculeatus | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 3.8 | optimally stable, stability declines sharply below pH 2.8 and above pH 5 | Aspergillus aculeatus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus aculeatus | pH gradient 3.5-9.5 | - |
3.4 |
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